Rice is one of the most important staple food in the world, and increasing rice production is crucial to the rapid growth of the global population. Grain size and weight are important yield and appearance quality traits, so optimizing grain size and weight is a key strategy to increase crop yield and improve appearance quality.
A joint team led by Prof. LI Yunhai at the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences, Prof. WANG Dekai at the Zhejiang Sci-Tech University, Profs. QIAN Qian and ZENG Dali at the China National Rice Research Institute and Prof. Dirk Inze at Ghent University recently revealed the GW2-WG1-OsbZIP47 pathway regulating rice grain size and weight which provides new insight on application of increasing crop yield and improving grain appearance quality.
First, they isolated WG1 gene from an original transgenic line WG1-OE with wider and heavier grains. WG1 encodes a CC-type glutaredoxin OsGRX8, which is localized in the nucleus. Upregulation of WG1 significantly increased grain width and weight, while knockout of WG1 using CRISPR/Cas9 technology lead to longer and thinner grains. Cytological analysis showed that WG1 regulates grain width by promoting cell proliferation.
WG1 interacts with the transcription factor OsbZIP47 and represses its transcriptional activity by recruiting the transcriptional co-repressor ASP1, so as to regulate grain size. OsbZIP47 restricts grain width growth by decreasing cell proliferation.
Further results reveal that a known grain size regulator-E3 ubiquitin ligase GW2 ubiquitinates WG1 proteins and targets them for degradation. Genetic analyses support that GW2, WG1 and OsbZIP47 function in a common pathway to control grain growth.
Thus, these findings reveal a genetic and molecular framework for the GW2-WG1-OsbZIP47 regulatory module-mediated control of grain size and weight, opening new perspectives for using this regulatory pathway for improvement of grain size and weight in crops.
This study, entitled “The GW2-WG1-OsbZIP47 pathway controls grain size and weight in rice” was published online in Molecular Plant on April 27 （DOI:10.1016/j.molp.2021.04.011）.
The research was supported by the National Natural Science Foundation of China and the strategic priority research program of the Chinese Academy of Sciences.
Model for the GW2-WG1-OsbZIP47 regulatory module-mediated control of grain width and weight (Image by Hao et al.)
Contact:
Dr. LI Yunhai
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
